cutsmart reaction buffer (New England Biolabs)
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cutsmart reaction buffer
Cutsmart Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cutsmart+buffer/T4+DNA+Ligase/pmc13674477-239-23-26
Average 99 stars, based on 33799 article reviews
Cutsmart Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cutsmart+buffer/T4+DNA+Ligase/pmc13674477-239-23-26
Average 99 stars, based on 33799 article reviews
cutsmart reaction buffer - by Bioz Stars,
2026-10
99/100 stars
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De-Phosphorylation Assay:Article Title: High-resolution mapping reveals features of bacterial NAD-capped RNAs and stress-responsive transcription initiation. Article Snippet: .. AR TI CL E IN P RE SS Dephosphorylation was carried out with 25 U of calf intestinal alkaline phosphatase (NEB, M0290) in 1× Article Title: High-resolution mapping reveals features of bacterial NAD-capped RNAs and stress-responsive transcription initiation. Article Snippet: RNA fragmentation was performed in 600 μL of 10 mM Tris-HCl (pH 7.4) and 10 mM ZnCl2 at 75°C for 2.5 min, immediately quenched with 20 μL of 0.5 M EDTA (pH 8.0), and purified by acid phenol:chloroform:isoamyl alcohol (PCI, Invitrogen) extraction followed by ethanol precipitation. .. AR TI CL E IN P RE SS ARTICLE IN PRESS Dephosphorylation was carried out with 25 U of calf intestinal alkaline phosphatase (NEB, M0290) in 1× Purification:Article Title: High-resolution mapping reveals features of bacterial NAD-capped RNAs and stress-responsive transcription initiation. Article Snippet: .. AR TI CL E IN P RE SS Dephosphorylation was carried out with 25 U of calf intestinal alkaline phosphatase (NEB, M0290) in 1× Article Title: High-resolution mapping reveals features of bacterial NAD-capped RNAs and stress-responsive transcription initiation. Article Snippet: RNA fragmentation was performed in 600 μL of 10 mM Tris-HCl (pH 7.4) and 10 mM ZnCl2 at 75°C for 2.5 min, immediately quenched with 20 μL of 0.5 M EDTA (pH 8.0), and purified by acid phenol:chloroform:isoamyl alcohol (PCI, Invitrogen) extraction followed by ethanol precipitation. .. AR TI CL E IN P RE SS ARTICLE IN PRESS Dephosphorylation was carried out with 25 U of calf intestinal alkaline phosphatase (NEB, M0290) in 1× Article Title: A bacterial-killing type IV secretion system as a hidden antifungal device Article Snippet: The proteins were purified with using Ni-NTA resin (GE Healthcare, China) and eluted by PBS buffer with 250 mM imidazole; after that, the proteins were concentrated with ultrafiltration tubes using PBS buffer. .. A 40-μl reaction mixture containing 200 ng of DNA and 1× Incubation:Article Title: A bacterial-killing type IV secretion system as a hidden antifungal device Article Snippet: The proteins were purified with using Ni-NTA resin (GE Healthcare, China) and eluted by PBS buffer with 250 mM imidazole; after that, the proteins were concentrated with ultrafiltration tubes using PBS buffer. .. A 40-μl reaction mixture containing 200 ng of DNA and 1× Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs Article Snippet: .. 5 μg/ml of this linear PCR-product was then incubated with 250 U/ml T4 DNA ligase (Thermo Scientific) in Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs. Article Snippet: Yeast trains were routinely grown at 30 ◦C and with shaking at 200 pm in yeast peptone dextrose broth (YPD): 20 g/l peptone, 10 /l yeast extract, and 20 g/l glucose, or in synthetic drop-out edium lacking histidine (SC-His) (Sigma –Aldrich, #Y1751). .. 5 μg/ml of this linear PCR-product was then incubated with 250 U/ml T4 DNA ligase (Thermo Scientific) in Positive Control:Article Title: A bacterial-killing type IV secretion system as a hidden antifungal device Article Snippet: The proteins were purified with using Ni-NTA resin (GE Healthcare, China) and eluted by PBS buffer with 250 mM imidazole; after that, the proteins were concentrated with ultrafiltration tubes using PBS buffer. .. A 40-μl reaction mixture containing 200 ng of DNA and 1× Polymerase Chain Reaction:Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs Article Snippet: .. 5 μg/ml of this linear PCR-product was then incubated with 250 U/ml T4 DNA ligase (Thermo Scientific) in Article Title: High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs. Article Snippet: Yeast trains were routinely grown at 30 ◦C and with shaking at 200 pm in yeast peptone dextrose broth (YPD): 20 g/l peptone, 10 /l yeast extract, and 20 g/l glucose, or in synthetic drop-out edium lacking histidine (SC-His) (Sigma –Aldrich, #Y1751). .. 5 μg/ml of this linear PCR-product was then incubated with 250 U/ml T4 DNA ligase (Thermo Scientific) in |